GUI freezing for second run

import sys
import os
from decimal import Decimal
import time
from PyQt5 import QtCore, QtGui, QtWidgets, uic
from PyQt5 import QtCore, QtGui, QtWidgets
from PyQt5.QtWidgets import *
from PyQt5.QtCore import *
from PyQt5.QtWidgets import QApplication, QWidget, QPushButton, QMessageBox
from pathlib import Path
from CONNECTDATABASE import*
from RB import*
from UPDATEDATABASEthread import*
import subprocess
from urllib.request import urlopen
qtCreatorFile = "SMOG_COLASCE_DEVELOPER_INTERFACE1.ui" # Enter file here.
Ui_MainWindow, QtBaseClass = uic.loadUiType(qtCreatorFile)
class Worker(QObject):
    hashed = pyqtSignal(str)
    signal=pyqtSignal(int)
    finished = pyqtSignal()
    @pyqtSlot(str,str,str,str,str,str)
    def cal_functions(self,filter,filter1,IND,OTD,Version,Year):
        if filter=='RB':
            if filter1 =='None':
                x=ARBthread(self,IND,OTD,Version,Year)
                self.hashed.emit(x)

        self.finished.emit()
class Worker1(QObject)   :
    def __init__(self):
        super().__init__()
    hashed1 = pyqtSignal(str)
    signal=pyqtSignal(int)
    finished = pyqtSignal()
    @pyqtSlot(str,str,str,str,str,str,str,str,str)
    def update_database(self,hostname,port,username,password,Database_Name,IND,OTD,Version,Year):
        x1=UPDATEDATABASEthread(self,hostname,port,username,password,Database_Name,IND,OTD,Version,Year)
        self.hashed1.emit(x1)
        self.finished.emit()

class MyWindow(QtWidgets.QMainWindow, Ui_MainWindow):
    cal_requested = pyqtSignal(str,str,str,str,str,str)
    data_requested= pyqtSignal(str,str,str,str,str,str,str,str,str)
    def __init__(self):
        QtWidgets.QMainWindow.__init__(self)
        Ui_MainWindow.__init__(self)
        self.setupUi(self)
        self.setWindowTitle("SMoG-India COALESCE")
        self.Sector.currentIndexChanged.connect(self.comboBox_sector_change)
self.SubSectorLevel1.currentIndexChanged.connect(self.comboBox_subsector_change)
        self.Calculate.clicked.connect(self.calc)
        self.toolButton.clicked.connect(self._open_file_dialog)
        self.toolButton_2.clicked.connect(self._open_file_dialog1)
        self.toolButton_3.clicked.connect(self._open_file_dialog2)
        self.checkBox_2.stateChanged.connect(self.checkedcLS)
        self.checkBox_3.stateChanged.connect(self.checkedcRS)
        self.pushButton_3.clicked.connect(self.Database)
        self.pushButton_4.clicked.connect(self.ConnectDatabase)
        self.lineEdit_5.setReadOnly(True)
        self.lineEdit_6.setReadOnly(True)
        self.lineEdit_7.setReadOnly(True)
        self.lineEdit_8.setReadOnly(True)
        self.lineEdit_9.setReadOnly(True)
        self.pushButton_2.clicked.connect(self.close_event)
        self.pushButton.clicked.connect(self.open_tutorial)

        # Create a worker object and a thread
        self.worker = Worker()
        self.worker_thread = QThread()
        self.worker.hashed.connect(self.messagebox)
        self.worker.signal.connect(self.update_progress_bar)
        self.cal_requested.connect(self.worker.cal_functions)
        self.worker.moveToThread(self.worker_thread)
        self.worker.finished.connect(self.worker_thread.quit)
        self.worker.finished.connect(self.worker.deleteLater)
        self.worker_thread.start()
        self.worker_thread.finished.connect(self.finish)
        self.worker1 = Worker1()
        self.worker1_thread = QThread()
        self.worker1.hashed1.connect(self.messagebox)
        self.worker1.signal.connect(self.update_progress_bar)
        self.worker1.moveToThread(self.worker1_thread)
        self.data_requested.connect(self.worker1.update_database)
        self.worker1.finished.connect(self.worker1_thread.quit)
        self.worker1.finished.connect(self.worker1.deleteLater)
        self.worker1_thread.finished.connect(self.worker1_thread.deleteLater)
        self.worker1_thread.start()
        self.worker1_thread.finished.connect(self.finish)
   def calc(self):
        progressBar = self.progressBar
        self.Calculate.setEnabled(False)
        self.SubSectorLevel1.setEnabled(False)
        self.SubSectorLevel2.setEnabled(False)
        self.Sector.setEnabled(False)
        self.toolButton_2.setEnabled(False)
        self.toolButton_3.setEnabled(False)
        self.lineEdit_10.setReadOnly(True)
        self.lineEdit_4.setReadOnly(True)
        self.toolButton_2.setEnabled(False)
        self.toolButton_3.setEnabled(False)
        filter = self.SubSectorLevel1.currentText()
        filter1 = self.SubSectorLevel2.currentText()
        IND = self.lineEdit.text()
        OTD = self.lineEdit_2.text()
        Version= self.lineEdit_10.text()
        Year=self.lineEdit_4.text()
        self.cal_requested.emit(filter,filter1,IND,OTD,Version,Year)